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Titre : | Knockdown of miR-299-5p inhibits the progression of hepatocellular carcinoma by targeting SIAH1 (2018) |
Auteurs : | Xinghua Jiang ; Xiaoxu Shen |
Type de document : | Article |
Dans : | Bulletin du cancer (Vol. 105, n° 10, Octobre 2018) |
Article en page(s) : | p. 873-883 |
Note générale : | Doi : 10.1016/j.bulcan.2018.07.013 |
Langues: | Français |
Descripteurs : |
HE Vinci Biologie cellulaire ; Carcinome hépatocellulaire ; Génétique ; Marqueurs biologiques ; MicroARN |
Mots-clés: | Seven in absentia proteins ; SIAH1 |
Résumé : |
BACKGROUND : Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide. MiR-299-5p has been demonstrated to play important roles in multiple human cancers. Nevertheless, little is known about the detailed function and molecular mechanism of miR-299-5p on HCC progression.
METHODS : Quantitative real-time PCR (qRT-PCR) assay was used to assess the expression patterns of miR-299-5p and siah E3 ubiquitin protein ligase 1 (SIAH1) in HCC tissues and cell lines. Loss-of-function experiments were performed to explore the effect of miR-299-5p on HCC progression in vitro and in vivo. Target predicted by software algorithms, the connection between miR-299-5p and SIAH1 was verified by dual-luciferase reporter assay, qRT-PCR and western blot analysis. Subsequently, anti-miR-299-5p and si-SIAH1 were cotransfected into LM9 and Huh-7 cells to further explore whether the regulatory effect of miR-299-5p on HCC was mediated by SIAH1. RESULTS : qRT-PCR assay revealed that miR-299-5p was upregulated and SIAH1 was downregulated in HCC tissues and cell lines. Moreover, miR-299-5p knockdown suppressed HCC progression in vitro and in vivo. In addition, anti-miR-299-5p-mediated regulatory effect on HCC cells was abated following the restoration of SIAH1 expression. CONCLUSIONS : MiR-299-5p knockdown suppressed the progression of HCC by targeting SIAH1, highlighting its role as a potential biomarker and therapeutic target of HCC. |
Disponible en ligne : | Non |
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