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Titre : | Essai d'automatisation en cytometrie 10 couleurs |
Auteurs : | Soufiane Gharbi, Auteur ; Danai Poutakidou, Promoteur |
Type de document : | Travail de fin d'études |
Editeur : | Woluwe-Saint-Lambert : Haute École Léonard de Vinci, 2022 |
Langues: | Français |
Index. décimale : | TFE - Biologie médicale |
Descripteurs : |
HE Vinci Antigene ; Cytométrie en flux ; Immunophénotypage ; Lymphocyte ; Phénotype |
Résumé : |
ClearLLab T tube allows a more precise diagnosis of T-cell neoplasms. A comparison is made between the ClearLab B tube and the B tube used at the LHUB-ULB, and between the ClearLab T tube and the T tube used in routine. The comparison shows whether similar proportions are obtained for the main lymphocyte phenotypes sought in the clinic, with the Beckman Coulter tubes and the tubes used at LHUB-ULB.
The study showed that the percentage of abnormal B cells was not significantly different (p = 0.9971) between the ClearLLab LS tube and the B tube used at LHUB-ULB. This comparison could not be made for T lymphocytes due to the absence of CD7 in the ClearLLab LS tube. For B lymphomas, the results are concordant (p > 0.05) between the ClearLLab B tube and the routine B tube for all markers except for the percentage of pathological CD20+ B cells, which is significantly lower in ClearLLab. This is particularly troublesome in cases of CD20dim lymphomas, where a significant proportion of the B lymphoma cells are falsely categorised as CD20neg. Regarding T abnormalities, the results are concordant between the ClearLLab T tube and the T tube used by the LHUB-ULB for all markers compared, except for CD7 and for the CD4/CD8 ratio. Indeed, a discordance is observed for these markers but only for healthy samples with p-values of 0.0403 and 0.0307 respectively. In conclusion, the ClearLLab LS tube is a good screening tube for B lymphocytes but it is not able to detect T lymphocytes with only aberrant CD7 expression, which is problematic. The ClearLLab T tube appears to be equivalent to the T tube used at LHUB-ULB but this needs to be confirmed. The ClearLLab B tube underestimates CD20 expression in CD20dim B lymphomas. |
Accès : | Identifiez-vous avant d'accéder au document électronique |
Disponible en ligne : | Oui |
Lieu du stage : | Laboratoire d'hematologie, LHUB - ULB |
Département : | Biologie médicale |
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TFE - Biologie médicale Adobe Acrobat PDF |