Login
Communauté Vinci
Extérieur
Si votre nom d'utilisateur ne se termine pas par @vinci.be ou @student.vinci.be, utilisez le formulaire ci-dessous pour accéder à votre compte de lecteur.
Titre : | Identification ultra-rapide des hémocultures positives dans un bouillon detsb & détermination du profil de sensibilité/résistance des entérobactéries aux céphalosporines de troisième génération par le module star cepha |
Auteurs : | Marie-Rose MESSY, Auteur ; Alexia Verroken, Promoteur |
Type de document : | Travail de fin d'études |
Editeur : | Bruxelles : Institut Paul Lambin, 2020 |
Langues: | Français |
Index. décimale : | TFE - Biologie médicale |
Résumé : | Bacteremia is defined as the presence of bacteria in the blood. Bacteremia or bloodstream infections are associated with high rates of morbidity and mortality. Therefore, the role of microbiology laboratories is the rapid identification of the germ responsible for the infection and the determination of its antibiotic susceptibility/resistance profile. This is why the study I have conducted is on: - the ultra-rapid identification of positive blood culture germs (ID UR HC+) from TSB (Tryptic Soy Broth). This technique consists in mixing 1mL of TSB with 5 drops of positive blood, then incubating them for 1 hour at 35°C, centrifuging them, then placing the pellet on a MALDI-TOF MS plate and proceeding with the identification. Once the results are obtained, compare them with the routine results. ET- Determination of the sensitivity/resistance profile of Enterobacteriaceae to C3G (third generation cephalosporin) on positive blood samples via the STAR CEPHA module. This technique consists in incubating Enterobacteriaceae with C3G for 30 min at 35°C. After incubation, 1μL of supernatant is deposited on a MALDI-TOF target plate. The results obtained (hydrolyzed antibiotic, non-hydrolyzed antibiotic or no results) in this technique are compared to the reference results (automated Phoenix system). The beta-lacta test technique already in routine use was also compared to the results of the Phoenix. This technique uses the same principle as the STAR-CEPHA, its involvement in this study is for comparison purposes only. The results obtained do not allow us to validate the URD technique because the number of vials with a lack of identification is high in the different types of vials. Furthermore, when we set a cut-off score of 1.7, the match rates are just under 50%. The results obtained from the STAR-CEPHA study, i.e. a sensitivity of 54.55%, a specificity of 90.91%, a positive predictive value of 60% and a negative predictive value of 88.89%, do not allow us to validate this technique because it has many false negatives that lead to low sensitivity. From this study, it appears that the ultra-fast identification technique is a technique that can substitute the current routine technique, but it still needs to be confirmed by other techniques. As for STAR-CEPHA, the results obtained at the end of this work do not allow us to validate this technique because it contains many erroneous and uncertain results. Therefore, the beta-lacta test technique remains the technique for determining the sensitivity/resistance profiles of Enterobacteriaceae to C3G. |
Accès : | Identifiez-vous avant d'accéder au document électronique |
Disponible en ligne : | Oui |
Lieu du stage : | Cliniques UniversitairesSaint Luc - CUSL(Bactériologie) |
Département : | Biologie médicale |
Documents numériques (1)
Ce document n'est visible qu'après identification
TFE biologie médicale Adobe Acrobat PDF |