| Titre : | Vérification d’une méthode de dosage du facteur XI |
| Auteurs : | Anouvong Vongsavath, Auteur ; Laurence Rozen, Promoteur |
| Type de document : | Travail de fin d'études |
| Editeur : | Woluwe-Saint-Lambert : Haute École Léonard de Vinci, 2024 |
| Index. décimale : | TFE - Biologie médicale |
| Mots-clés: | Hémostase ; Coagulation ; Facteur XI ; Facteur de Rosenthal ; Automate ; Dosage ; Voie intrinsèque ; Vérification de méthode |
| Résumé : | Vérification de la méthode de dosage du facteur XI par l’automate de CN-6000 de sysmex. Haemostasis is a complex mechanism with the purpose of forming a platelet clot in order to stop the bleeding during a vascular breach. Several actors will intervene during this process, such as the clotting factors. The factor XI is one of them and the subject of this work. The most known hemophilias, are hemophilia A and B, caused by a genetic deficiency of the factor VIII or IX respectively. These diseases are linked to a recessive X-chromosome. There also exist a hemophilia C, caused by a deficiency of the factor XI. Unlike the first mentioned hemophilias, hemophilia C, also named Rosenthal disease, is transmited by an autosomal chromosome. The purpose of this work is to verify the factor XI quantification method of the CN-6000 analyser, created by Sysmex, in order to be approved for accreditation and the replacement of the old CS-2500 analyser. Measurements comparison between CN-6000 and CS-2500 analysers, repeatability, reproducibility, limits of quantification, contaminations are the different parameters evaluated. The previously mentioned parameters yielded results in accordance with the current requirement and standards or those set by the laboratory with the notable exception of contamination tests. The examined contaminations are the inter-sample and the inter-reagent contaminations. While the former showed no contamination, the later suggested possible contamination between reagents, in particular, between those of standard analysis and those of clotting factor. However, the procedure set by the laboratory state that no quantification of clotting factor will be realised at the same time of a standard analysis. Hence, no such contamination should happen. The upper limit of quantification has been set to 160 % and the lower one to 1,5 %. The measurements comparison of the two analysers showed no significant differences and are therefore interchangeable. The last parameters, such as measurement uncertainty or interferences, will be examined in 2024. The accreditation file will be submited the same year. |
| Disponible en ligne : | Oui |
| Lieu du stage : | LHUB-ULB site Horta, Van Gehuchten 4, 1020 laboratoire d’hématologie, Square Brussels |
| Département du TFE : | Biologie médicale |
Bibliothèques HE Vinci
