| Titre : | Perspectives d'optimisations autour de 2 textes du bilan d'infertitlité |
| Auteurs : | Mohamed Aziz Raoudi, Auteur ; Damien Gruson, Promoteur |
| Type de document : | Travail de fin d'études |
| Editeur : | Woluwe-Saint-Lambert : Haute École Léonard de Vinci, 2024 |
| Index. décimale : | TFE - Biologie médicale |
| Mots-clés: | Infertilité ; Hormone Anti-Mullérienne ; Testostérone ; Développement durable |
| Résumé : |
Purpose and Problem Statement : This study tackles the challenges in measuring hormones accurately, which is crucial for diagnosing infertility issues. As testing technologies improve, it’s important to test and compare new methods with routine ones to increase the accuracy of diagnoses, lessen environmental effects, and improve lab operations.
Methods : This study used the Maglumi® X6 chemiluminescence immunoassay for analyzing free testosterone and a traditional radioimmunoassay (RIA). The accuracy of the Maglumi® X6 was thoroughly tested for consistency and reliability using the CLSI EP15-A3 protocol and Westgard rules. It also used the AnshLabs Ultra-Sensitive AMH/MIS ELISA kit for both manual and automated methods on the Euroimmun Analyzer I for analyzing Anti-M¨ullerian Hormone (AMH). The study also looked at the environmental sustainability of RIA and CLIA methods, and a survey was conducted to evaluate lab practices. Results : The Maglumi® X6 showed equal or better accuracy than the traditional RIA for testing free testosterone, with significant improvements in safety and environmental effects. Consistency and reliability tests confirmed that the Maglumi® X6 meets high-quality standards. The ELISA analysis for AMH showed highly accurate and consistent results. Additionally, the survey on eco-responsibility revealed areas for further reducing environmental impacts in lab settings. Conclusions : Moving from RIA to CLIA for hormone testing, especially with the Maglumi® X6, greatly improves safety, environmental impact, and diagnostic reliability. These improvements align with current demands for more sustainable lab practices without compromising the quality of patient care. Future work should extend these findings to larger groups of patients and various biological markers to support the broader use of CLIA technologies, balancing innovation with environmental and clinical excellence. |
| Disponible en ligne : | Oui |
| Lieu du stage : | Cliniques universitaires Saint-Luc (UCLouvain) |
| Département du TFE : | Biologie médicale |
Bibliothèques HE Vinci
