| Titre : | Mise au point de contrôles internes utiles à l’analyse métagénomique de microbiotes |
| Auteurs : | Lysa PINSMAYE, Auteur ; Jean Ruelle, Promoteur |
| Type de document : | Travail de fin d'études |
| Editeur : | Bruxelles : Institut Paul Lambin, 2020 |
| Langues: | Français |
| Index. décimale : | TFE - Biologie médicale |
| Résumé : |
As an introduction, filamentous fungi are heterotrophic eukaryotic organisms, which feed themselves by absorption of carbon source either by saprophytism if they develop on an inert material or by parasitism if they develop on a living. They spread very easily and can be the cause of serious pathologies, especially in immunocompromised patients with infections by opportunistic fungi and in healthy people exposed to high concentrations of fungi. The rapid detection of a fungal infection is essential to avoid the risks of mortality, especially in immunocompromised people.
Unlike bacteria and yeasts, the identification by MALDI-TOF is not yet used for filamentous fungi in routine due to the taxonomy of species, which evolve, with the arrival of sequencing methods allowing discriminating species complexes, which are difficult to discriminate with routine methods. The aim of this research is to evaluate the identification of filamentous fungi by rapid procedures and based on the analysis of mass spectra by Maldi-Tof from growth on PDA and ID-FP medium. For this, the analyzes were carried out on different kinds of fungi from pure reference strains and identified by the laboratory through sequencing. The study was carried out on 10 species of Aspergillus, 11 species of Mucorals, 19 other genera of Hyphomycetes and 23 species of dermatophytes by different rapid procedures starting from growth on ID-FP medium compared to a PDA medium used in routine with variable incubations times. The results show that young cultures of 24h incubation give higher identification scores for the genius Aspergillus while the Mucorals and Hyphomycetes show an improvement of the scores on cultures of 48h of incubation from ID-FP medium. For dermatophytes, incubation times vary from 24 hours to 16 days depending on the species. Finally, the media used and the incubation conditions influence the identification results, however the identifications are sometimes not exact up to the species and don’t give reliable results in the majority of cases which is due to availability of reference spectra in the library of the MBT Bruker database. |
| Accès : | Identifiez-vous avant d'accéder au document électronique |
| Disponible en ligne : | Oui |
| Lieu du stage : | Pôle de Microbiologie médicale - Institut de Recherche Expérimentale et Clinique - UCLouvain |
| Département du TFE : | Biologie médicale |
Documents numériques (1)
 TFE biologie médicale Adobe Acrobat PDF |
