| Titre : | Stress oxydatif et inflammatoire induits par le H2O2 et le LPS: les effets d’une cyclodextrine (HPbCD) et d’un anti-inflammatoire glucocorticoïde (Budésonid) |
| Auteurs : | Léna KAMGA MAGNE, Auteur ; Marie-Paule Mingeot-Leclercq, Promoteur |
| Type de document : | Travail de fin d'études |
| Editeur : | Bruxelles : Institut Paul Lambin, 2020 |
| Langues: | Français |
| Index. décimale : | TFE - Biologie médicale |
| Résumé : |
Chronic obstructive pulmonary disease (COPD) is a respiratory disease characterized by constriction and inflammation of the bronchi, it’s mainly caused by smoking. According to the WHO, it’s the 5th leading cause of death in the world. In fact, it affects adults over 40 years old and increases with age. The main treatments are based on bronchodilators and anti-inflammatory drugs which reduce the frequency of attacks, symptoms, and the severity of exacerbations. There is no curative treatment for COPD to date. Many patients do not respond or no longer to treatment with corticosteroids and develop corticosteroid resistance.
To this end, researchers from the University of Liege have suggested a potential interest for budesonide complexed with a cyclodextrin, hydroxypropyl-β-cyclodextrin (BUD: HPbCD) as an alternative treatment for COPD. Following this, researchers from the catholic University of Louvain carried out experiments using in vitro human cell models, A549 cells (pulmonary alveolar cells) and THP-1 cells (cells of the monocytic line). Based on these experiences, it emerged that the complex (BUD: HPbCD) would have an inhibitory effect on oxidative stress and inflammatory stress induced by the mixture H2O2+ LPS by mechanisms involving the lipid domains of the membranes of lung cells. The aims of this work was to compare the effects of the complex (BUD: HPbCD) and the association (BUD + HPbCD) of the two constituents uncomplexed on oxidative stress induced by the mixture of H2O2+ LPS in A549 cells and the inflammatory stress induced by the LPS in THP-1 cells made insensitive to the anti- inflammatory glucocorticoids by H2O2 pretreatment. In conclusion, the complex (BUD: HPbCD) and the uncomplexed association (BUD+ HPbCD) appear to have the same effects and are able to decrease the oxidative stress and inflammatory stress induced by H2O2 and LPS in cellular models. |
| Accès : | Identifiez-vous avant d'accéder au document électronique |
| Disponible en ligne : | Oui |
| Lieu du stage : | Louvain Drug Research Institute (LDRI)/UCLouvain - Unité de Pharmacologie cellulaire et moléculaire |
| Département du TFE : | Biologie médicale |
Documents numériques (1)
 TFE biologie médicale Adobe Acrobat PDF |
