| Titre : | Recolonisation de vaisseaux décellularisés et de matrices d’organes décellularisées et vascularisées. |
| Auteurs : | Camille DETRY, Auteur ; C. Behets, Promoteur ; Louis MAISTRIAUX, Promoteur |
| Type de document : | Travail de fin d'études |
| Editeur : | Bruxelles : Institut Paul Lambin, 2020 |
| Langues: | Français |
| Index. décimale : | TFE - Biologie médicale |
| Résumé : |
Organ transplantation is still a short-term solution for patients. There is a high risk of transplant rejection, due to an imperfect immunological match, which leads to the death of the graft or the patient. Tissue engineering is then used as a solution.
Tissue engineering consists in producing a compatible organ by replacing the donor cells with the recipient's cells and thus obtaining a graft composed solely of the patient's cells, and perfectly compatible. This thesis will focus on endothelial cells, their studies and characteristics, with the aim of regenerating decellularized vessels and ultimately regenerating decellularized vascularized organs. Firstly, endothelial cells were harvested from porcine aorta and spleen pieces via four protocols; three of them showed convincing results. The cells were then cultured and isolated using either Dynabeads, preplating or both. In this way, it was possible to determine the appropriate protocol for harvesting the cells, keeping them alive and isolating them. Secondly, endothelial cells were characterized by immunofluorescence labelling with anti-CD31 antibodies. This made it possible to visualize the cells and to determine the rate of cells that had dedifferentiated, as well as the quality of the isolation. Thirdly, industrial hAECs cells were used to recolonize acellular heart valve matrices. Tests were then conducted over 3 successive days to determine the viability of the cells. This part is still in its early stages, the protocol still needs to be refined, but the results are encouraging. In conclusion, some protocols gave good results, while others were too tedious and not conclusive enough. Recolonization by industrial cells seems promising and makes it possible to envisage recolonization by primary cells in the near future. |
| Accès : | Identifiez-vous avant d'accéder au document électronique |
| Disponible en ligne : | Oui |
| Lieu du stage : | Pôle de Morphologie – UCLouvain |
| Département du TFE : | Biologie médicale |
Documents numériques (1)
 TFE biologie médicale Adobe Acrobat PDF |
